Leukocytes respiratory burst and lysozyme level in pacu (Piaractus mesopotamicus Holmberg, 1887).
نویسندگان
چکیده
Innate immune responses are useful to determine the health status of fish and to evaluate the effect of immunomodulatory substances in fish farming. Leukocytes respiratory burst was measured in pacu (Piaractus mesopotamicus) using chemiluminescence assay and nitroblue tetrazolium (NBT) reduction assay. The nitroblue tetrazolium reduction seemed more adequate than chemiluminescence assay for leukocytes oxidative burst determination, since it was difficult to isolate the blood leucocytes for chemiluminescence assay. Plasma and serum lysozyme were measured using a turbidimetric assay. The heating of serum and plasma samples (56 masculineC for 30 minutes) for complement system inactivation darkened the plasma samples and interfered in the results. The lysozyme activity in serum was higher than in plasma, suggesting that serum samples are more appropriate for the analysis. This study established protocols that can be useful tools in the study of immune mechanisms of the tropical fish pacu.
منابع مشابه
Structural and morphological features of Piaractus mesopotamicus (Holmberg, 1887) gills.
The pacu Piaractus mesopotamicus (Holmberg, 1887), is a commercially important serrasalmid fish endemic to the Paraná-Paraguay River basin, and one of the most widely cultivated native fish species in the Neotropics. As a migratory species, also inhabiting temporary pools subjected to hypoxic conditions, the species presents a high plasticity concerning respiratory adaptations. In order to supp...
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ورودعنوان ژورنال:
- Brazilian journal of biology = Revista brasleira de biologia
دوره 69 4 شماره
صفحات -
تاریخ انتشار 2009